- ≥95% by SDS-PAGE and HPLC
- Lot-matched certificate of analysis
- Ships next business day, tracked
ACE-031
Soluble ActRIIB-Fc fusion protein studied as a myostatin ligand trap
- FormLyophilized recombinant protein, sealed glass vial
- Research areasMyostatin-pathway inhibition, muscle-mass regulation, muscular dystrophy models, bone density models
Overview
ACE-031 is a recombinant fusion protein built from the extracellular ligand-binding domain of the human activin receptor type IIB (ActRIIB) joined to the Fc region of human IgG1. Rather than blocking a receptor on the cell surface, it works as a decoy: circulating myostatin, activin A and several related TGF-β superfamily ligands bind the soluble receptor domain and are removed from the pool available to signal through membrane-bound ActRIIB. The Fc portion dimerises the construct and extends its circulating half-life considerably compared with the receptor domain alone.
Ligand breadth is the property that decides where the construct is useful. The soluble ActRIIB domain binds not only myostatin but activin A, GDF-11 and several bone morphogenetic proteins, so an observed phenotype cannot be attributed to myostatin blockade alone without parallel arms using more selective reagents, which is why the myostatin propeptide and follistatin-344 are listed beside it. The material is a recombinant protein rather than a synthetic peptide, released to a 95% specification by SDS-PAGE and HPLC rather than the 99% HPLC figure quoted for short sequences, and it behaves like one: the disulfide-linked Fc homodimer aggregates under vigorous mixing and repeated freeze–thaw, so reconstitution is gentle, dilute stocks carry a carrier protein, and the single 1 mg vial is aliquoted before freezing.
Specifications
Supply & purity
- Form
- Lyophilized recombinant protein, sealed glass vial
- Purity
- ≥95% by SDS-PAGE and HPLC; lot-matched COA available
- Available sizes
- 1 mg
- Solubility
- Sterile or bacteriostatic water; carrier protein often added for dilute stocks
Handling & storage
- Storage (lyophilized)
- −20 °C, protected from light and moisture
- Storage (reconstituted)
- 2–8 °C for immediate work; aliquot and freeze for longer studies
Additional detail
- Protein class
- Fc-fusion protein; soluble activin receptor type IIB decoy
- Construct
- Extracellular domain of human ActRIIB fused to human IgG1 Fc
- Quaternary structure
- Disulfide-linked homodimer, as with other IgG1 Fc fusions
- Principal binding partners
- Myostatin (GDF-8), activin A, GDF-11 and selected BMPs
- Also known as
- ActRIIB-Fc, soluble activin receptor type IIB fusion protein, ramatercept
- Research areas
- Myostatin-pathway inhibition, muscle-mass regulation, muscular dystrophy models, bone density models
Working out the concentration
The vial sizes above — 1 mg — describe a mass of material, not a strength. Choose a diluent volume, work it out with the reconstitution calculator, and you have the concentration for your own vial of ACE-031 plus the volume that carries any mass you want to withdraw.
Certificate of analysis
Every lot of ACE-031 is released against a certificate of analysis: the batch-specific laboratory data for that lot, not a generic document for the compound. The lot number on the certificate matches the lot number printed on the label you receive — the vial label itself, where the product is vialled — so the record and the material it describes can always be reconciled.
Purity: ≥95% by SDS-PAGE and HPLC — the specification for this listing. The figure for the lot you receive is the one reported on that lot’s certificate.
No lot certificate is on display on this page at the moment. That is a publishing state and not a testing one: lots are released and documented as described above whether or not the document is posted here, and we will send what exists for the lot in stock, or for a lot already on your bench, by its number.
Certificates are available on request before you order as well as after, with no account and no charge. Request the certificate for this listing, read the testing and documentation policy in full, or see how to read a peptide COA.
Questions about ACE-031
What exactly is ACE-031?
It is a recombinant fusion protein pairing the extracellular ligand-binding domain of human activin receptor type IIB with the Fc region of human IgG1. The soluble receptor domain captures myostatin and related ligands, and the Fc portion dimerises the construct and extends its persistence in circulation.
How does ACE-031 differ from Follistatin-344?
Both intercept ligands in the same signalling axis but from different directions. Follistatin is a naturally occurring binding protein that wraps activin and myostatin directly, while ACE-031 is an engineered soluble copy of the receptor itself. They are often run as parallel arms in the same study.
Is your ACE-031 third-party tested?
Yes. Each lot is analysed by an independent laboratory, with purity assessed by SDS-PAGE and HPLC and identity confirmed by appropriate protein methods. The certificate of analysis is matched to your vial's lot number and available on request.
How should ACE-031 be stored?
Keep sealed lyophilized vials at −20 °C, protected from light and moisture. After reconstitution, hold at 2–8 °C for immediate work or aliquot and freeze for longer studies. Avoid repeated freeze–thaw cycles and vigorous mixing, both of which promote aggregation in Fc-fusion proteins.
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