Adipotide (FTPP) Peptide

Adipotide, also written FTPP (fat-targeted proapoptotic peptide), is a synthetic peptidomimetic built from two functional halves joined into a single molecule. One half is a cyclic nine-residue homing motif, CKGGRAKDC, identified by phage-display screening as a binder of a prohibitin-1 / annexin A2 complex reported on the endothelium of white adipose tissue. The other half is D(KLAKLAK)2, an amphipathic sequence assembled from D-amino acids that disrupts mitochondrial membranes once it reaches the inside of a cell. The design intent described in the literature is address-plus-payload: the homing motif supplies vascular selectivity, the effector domain supplies the activity.

The effector half is worth understanding on its own terms. D(KLAKLAK)2 is an amphipathic sequence built entirely from D-amino acids, which makes it protease-resistant while leaving its activity intact, because membrane disruption depends on the amphipathic helix rather than on a stereospecific receptor contact; it is comparatively inactive outside cells and acts once internalised, which is what makes it usable as a payload. Published work with the conjugate spans phage-display mapping of the homing motif, prohibitin biology, and rodent models of adipose vascular targeting, alongside the wider tumour-homing literature the same design strategy came from. Fills are 2 mg and 5 mg, and the stated mass near 2611 g/mol shifts with trifluoroacetate content, which varies by lot and bears on any molar calculation.

Reconstitution is where the arithmetic lives. The same vial made up with 1 mL or with 3 mL of diluent gives three-fold different numbers, which is why a reconstitution volume calculator is more use here than a fixed table: enter the vial mass and your diluent volume and read the concentration back.

For work specified in µM or nM rather than mg/mL, feed ≈2611.20 g/mol (trifluoroacetate salt content varies by lot) and your volume into the peptide molarity calculator.