- ≥98% by HPLC
- Lot-matched certificate of analysis
- Ships next business day, tracked
Metabolic & Circadian Compounds
Cardarine (GW-501516)
Non-peptide PPARδ agonist research chemical, capsules or 20 mg/mL liquid
- FormOral capsules in a sealed bottle, or 30 mL solution in an amber dropper bottle
- Mol. weight453.50 g/mol
- Research areasFatty-acid oxidation and PDK4/CPT1 expression, muscle fibre-type gene programmes, lipid and lipoprotein handling, anti-doping detection chemistry
Overview
GW-501516, widely catalogued as Cardarine and sometimes as GW1516 or endurobol, is a synthetic small molecule that acts as a high-affinity agonist at peroxisome proliferator-activated receptor delta (PPARδ, also written PPARβ/δ). It is not a peptide and not a SARM: structurally it is a thiazole-containing phenoxyacetic acid bearing a trifluoromethylphenyl group, with a molecular weight of roughly 453.5 g/mol.
PPARdelta operates as a ligand-activated transcription factor, heterodimerising with the retinoid X receptor and binding peroxisome proliferator response elements in target promoters, so the readouts in this literature are largely gene-expression ones. Studies quantify PDK4 and CPT1 transcript levels, fatty-acid oxidation rates, muscle fibre-type gene programmes and lipid and lipoprotein handling, in myotube culture and in rodent models. A second and entirely separate body of work is analytical: validated LC-MS/MS methods for the parent compound and its sulfoxide and sulfone metabolites, developed for doping control, where the compound is classified among metabolic modulators. Both formats here draw on the same material tested to at least 98% by HPLC, as sixty 10 mg capsules or as 30 mL of solution at 20 mg/mL for volumetric work.
Specifications
Identity
- CAS number
- 317318-70-0
- Molecular formula
- C21H18F3NO3S2
- Molecular weight
- 453.50 g/mol
Supply & purity
- Form
- Oral capsules in a sealed bottle, or 30 mL solution in an amber dropper bottle
- Purity
- ≥98% by HPLC; lot-matched COA available
- Available sizes
- 10 mg × 60 capsules; 30 mL liquid at 20 mg/mL
- Solubility
- Poorly water soluble; typically dissolved in DMSO or ethanol for in-vitro work
Additional detail
- Compound class
- Non-steroidal PPARδ (PPARβ/δ) agonist; nuclear receptor ligand
- Also known as
- Cardarine, GW1516, GSK-516, endurobol
- Chemical description
- Phenoxyacetic acid linked through a thioether to a 4-methyl-2-[4-(trifluoromethyl)phenyl]thiazole
- Molecular target
- PPARδ; acts with RXR as a heterodimeric transcription factor
- Research areas
- Fatty-acid oxidation and PDK4/CPT1 expression, muscle fibre-type gene programmes, lipid and lipoprotein handling, anti-doping detection chemistry
- Regulatory status
- Not an approved drug; not a dietary supplement or dietary ingredient; WADA prohibited (S4 metabolic modulators)
- Storage
- Room temperature in the sealed container, dry and protected from light; refrigerate stock solutions
Units and conversions
Capsule and troche strengths are stated per unit, so the figure a method needs is usually a conversion rather than a dilution. The mcg to mg converter handles the milligram-to-microgram step, and the millilitre arithmetic if you are working from a solution format instead.
Certificate of analysis
Every lot of Cardarine (GW-501516) is released against a certificate of analysis: the batch-specific laboratory data for that lot, not a generic document for the compound. The lot number on the certificate matches the lot number printed on the label you receive — the vial label itself, where the product is vialled — so the record and the material it describes can always be reconciled.
Purity: ≥98% by HPLC — the specification for this listing. The figure for the lot you receive is the one reported on that lot’s certificate.
No lot certificate is on display on this page at the moment. That is a publishing state and not a testing one: lots are released and documented as described above whether or not the document is posted here, and we will send what exists for the lot in stock, or for a lot already on your bench, by its number.
Certificates are available on request before you order as well as after, with no account and no charge. Request the certificate for this listing, read the testing and documentation policy in full, or see how to read a peptide COA.
Questions about Cardarine (GW-501516)
Is Cardarine a SARM?
No. Despite being sold alongside them, GW-501516 does not touch the androgen receptor. It is a PPARδ agonist — a nuclear receptor ligand that alters gene transcription in metabolic pathways. Grouping it with SARMs is a marketing convention, not a pharmacological one.
What purity and documentation are supplied?
Each lot is analysed by HPLC to at least 98% purity with identity confirmation, and a lot-matched certificate of analysis is available on request. Independent testing of consumer products in this category has often found mislabelled content, which is exactly why lot-level documentation matters.
How is it prepared for in-vitro work?
GW-501516 is poorly soluble in water. Cell-based studies generally prepare a concentrated stock in DMSO or ethanol and dilute into culture medium, keeping the final vehicle concentration low and matched in control wells. Stock solutions are usually stored cold and protected from light.
Is it detectable in anti-doping testing?
Yes. It is listed by WADA as a prohibited metabolic modulator, and validated LC-MS/MS assays detect both the parent compound and its sulfoxide and sulfone metabolites. Much of the analytical literature on this molecule exists for that purpose.
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