- ≥99% by HPLC
- Lot-matched certificate of analysis
- Ships next business day, tracked
GHK Basic (Tripeptide-1)
Copper-free Gly-His-Lys tripeptide for matrix and fibroblast research
- FormWhite to off-white lyophilized powder, sealed glass vial
- Mol. weight340.38 g/mol
- Research areasFibroblast signaling, matrix turnover, copper coordination, protease regulation and reactive-carbonyl chemistry
Overview
GHK Basic is the copper-free tripeptide glycyl-L-histidyl-L-lysine, also called Tripeptide-1. The distinction from GHK-Cu is chemical rather than cosmetic: GHK Basic contains the three-amino-acid ligand by itself, while GHK-Cu is a coordinated copper(II) complex with different mass, color and redox behavior. The uncomplexed peptide is a white to off-white powder rather than the characteristic blue of the copper form. Cosmetic-ingredient nomenclature keeps the same split, listing the free peptide as Tripeptide-1 and the complex as Copper Tripeptide-1, which is worth checking on supplier paperwork before two datasets are compared.
GHK occurs naturally in human plasma and also appears as a sequence within extracellular-matrix proteins; it was first described in the early 1970s as a low-molecular-weight activity separated from human plasma. Cell and biochemical research has examined its copper-binding equilibrium, fibroblast signaling, extracellular-matrix gene expression, protease regulation and reactive-carbonyl chemistry. Because trace copper in media can convert part of a nominally copper-free preparation into GHK-Cu, careful studies measure or control metal content.
Specifications
Identity
- CAS number
- 49557-75-7
- Molecular formula
- C14H24N6O4
- Molecular weight
- 340.38 g/mol
- Amino acid sequence
- Gly-His-Lys (GHK)
- Peptide class
- Endogenous matrix-associated tripeptide and copper-binding ligand
Supply & purity
- Form
- White to off-white lyophilized powder, sealed glass vial
- Purity
- ≥99% by HPLC; lot-matched COA available
- Available sizes
- 50 mg, 200 mg
- Solubility
- Water-soluble; use metal-controlled buffers for copper-comparison studies
Additional detail
- Also known as
- Tripeptide-1, glycyl-L-histidyl-L-lysine, GHK free peptide
- Copper status
- No coordinated copper added; trace-metal conditions remain an assay variable
- Research areas
- Fibroblast signaling, matrix turnover, copper coordination, protease regulation and reactive-carbonyl chemistry
- Storage
- −20 °C lyophilized; protect from moisture and use refrigerated solutions within the study window
Working out the concentration
The vial sizes above — 50 mg, 200 mg — describe a mass of material, not a strength. Choose a diluent volume, work it out with the reconstitution calculator, and you have the concentration for your own vial of GHK Basic (Tripeptide-1) plus the volume that carries any mass you want to withdraw.
For work specified in µM or nM rather than mg/mL, feed 340.38 g/mol and your volume into the peptide molarity calculator.
Certificate of analysis
Every lot of GHK Basic (Tripeptide-1) is released against a certificate of analysis: the batch-specific laboratory data for that lot, not a generic document for the compound. The lot number on the certificate matches the lot number printed on the label you receive — the vial label itself, where the product is vialled — so the record and the material it describes can always be reconciled.
Purity: ≥99% by HPLC — the specification for this listing. The figure for the lot you receive is the one reported on that lot’s certificate.
No lot certificate is on display on this page at the moment. That is a publishing state and not a testing one: lots are released and documented as described above whether or not the document is posted here, and we will send what exists for the lot in stock, or for a lot already on your bench, by its number.
Certificates are available on request before you order as well as after, with no account and no charge. Request the certificate for this listing, read the testing and documentation policy in full, or see how to read a peptide COA.
Questions about GHK Basic (Tripeptide-1)
Is GHK Basic the same as GHK-Cu?
No. GHK Basic is the copper-free Gly-His-Lys ligand. GHK-Cu is the coordinated copper(II) complex, which has a different molecular mass, blue color and redox chemistry. They need separate controls and should not share one product identity.
What is Tripeptide-1?
Tripeptide-1 is the cosmetic and catalog name commonly used for glycyl-L-histidyl-L-lysine, abbreviated GHK. The product here is the free peptide with sequence Gly-His-Lys and CAS 49557-75-7.
Why compare GHK Basic with Pal-GHK?
Pal-GHK adds a sixteen-carbon palmitoyl group to the same peptide core. That lipid tail changes solubility, membrane partitioning and surface adsorption. Running both forms helps isolate the effect of lipidation from the underlying GHK sequence.
What purity and documentation are provided?
The material is released at ≥99% purity by reversed-phase HPLC, and a certificate of analysis matched to the vial's lot is available. Studies focused on copper speciation should perform their own metal analysis as well.
What sizes are available?
GHK Basic is listed in 50 mg and 200 mg sealed research vials. Those are the size points observed in current specialist peptide catalogs and support both cell-culture and formulation-development work.
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