- ≥99% by HPLC
- Lot-matched certificate of analysis
- Ships next business day, tracked
NAD+
Nicotinamide adenine dinucleotide coenzyme, 500 mg and 1000 mg vials
- FormLyophilized powder, sealed glass vial
- Mol. weight663.43 g/mol
- Research areasCellular redox state, DNA repair signaling, mitochondrial metabolism, aging biology
Overview
NAD+ (nicotinamide adenine dinucleotide, oxidized form) is a dinucleotide coenzyme rather than a peptide. It consists of nicotinamide mononucleotide joined to adenosine monophosphate through a pyrophosphate bridge, and it is present in every living cell as the central electron carrier of catabolic metabolism. In its oxidized state it accepts a hydride to become NADH; the ratio between the two forms is one of the most-measured indicators of cellular redox state.
Fills here are larger than a peptide vial by one or two orders of magnitude — 100 mg, 500 mg and 1000 mg, plus a five-vial kit of 500 mg — because the enzymology consumes the coenzyme rather than merely using it catalytically. Sirtuins, the PARP family and the CD38 ectoenzymes all cleave NAD+ at its glycosidic bond and release nicotinamide, so each turnover destroys a molecule. At 663.43 g/mol, 500 mg amounts to about 754 micromoles, which suits assays run at the micromolar to millimolar concentrations these enzymes require. The dinucleotide is freely water-soluble, but its solutions are acidic and hydrolyse readily above neutral pH, so preparations are buffered, kept cold and used promptly rather than stored.
Specifications
Identity
- CAS number
- 53-84-9
- Molecular formula
- C21H27N7O14P2
- Molecular weight
- 663.43 g/mol
Supply & purity
- Form
- Lyophilized powder, sealed glass vial
- Purity
- ≥99% by HPLC; lot-matched COA
- Available sizes
- 100 mg, 500 mg, 1000 mg
- Solubility
- Freely soluble in water; solutions are acidic and best buffered
Handling & storage
- Storage (lyophilized)
- −20 °C, protected from light and moisture
- Storage (reconstituted)
- 2–8 °C, used promptly; NAD+ hydrolyzes at alkaline pH
Additional detail
- Chemical class
- Dinucleotide coenzyme (free acid form)
- Also known as
- Nicotinamide adenine dinucleotide, oxidized NAD, DPN
- Enzyme substrate for
- Sirtuins (SIRT1–7), PARP family, CD38/CD157
- Research areas
- Cellular redox state, DNA repair signaling, mitochondrial metabolism, aging biology
Working out the concentration
A lyophilized vial carries a mass on the label and no concentration anywhere, so the first calculation of any protocol is the reconstitution one. The peptide concentration calculator converts vial mass plus diluent volume into mg/mL, and back into the draw volume for a given mass.
Where a method is written in molar terms instead, the molecular weight above — 663.43 g/mol — is the input the molarity calculator needs.
Certificate of analysis
Every lot of NAD+ is released against a certificate of analysis: the batch-specific laboratory data for that lot, not a generic document for the compound. The lot number on the certificate matches the lot number printed on the label you receive — the vial label itself, where the product is vialled — so the record and the material it describes can always be reconciled.
Purity: ≥99% by HPLC — the specification for this listing. The figure for the lot you receive is the one reported on that lot’s certificate.
No lot certificate is on display on this page at the moment. That is a publishing state and not a testing one: lots are released and documented as described above whether or not the document is posted here, and we will send what exists for the lot in stock, or for a lot already on your bench, by its number.
Certificates are available on request before you order as well as after, with no account and no charge. Request the certificate for this listing, read the testing and documentation policy in full, or see how to read a peptide COA.
Questions about NAD+
Is NAD+ a peptide?
No. NAD+ is a dinucleotide coenzyme built from nicotinamide mononucleotide and adenosine monophosphate joined by a pyrophosphate bridge. It is catalogued alongside research peptides because laboratories studying mitochondrial and aging biology commonly use both.
What is the difference between NAD+ and NADH?
They are the oxidized and reduced forms of the same coenzyme. NAD+ accepts a hydride to become NADH during catabolic reactions, and the ratio between them is used as a readout of cellular redox state in metabolic research.
Is this NAD+ third-party tested?
Yes. Every lot is analyzed by HPLC for purity of ≥99%, and a lot-matched Certificate of Analysis is available on this page before purchase so you can confirm identity and purity against your own acceptance criteria.
How should NAD+ solutions be handled?
Dissolve in water or a suitable buffer, keep the pH near or below neutral, and use solutions promptly. NAD+ hydrolyzes under alkaline conditions and degrades with warmth, so cold storage, light protection and aliquoting all help preserve assay-grade material.
How does NAD+ compare with NMN or nicotinamide riboside?
NMN and nicotinamide riboside are precursors that cells convert into NAD+ through the salvage pathway, whereas NAD+ is the intact coenzyme. Comparative studies of precursor loading versus direct coenzyme supply are an active research topic.
What sizes are offered?
NAD+ is supplied in 500 mg and 1000 mg lyophilized vials. Larger quantities than a typical peptide are usual because enzyme assays and cell-culture work consume the coenzyme at millimolar to micromolar concentrations.
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