- ≥99% by HPLC
- Lot-matched certificate of analysis
- Ships next business day, tracked
Tripeptide-29 (Gly-Pro-Hyp)
Gly-Pro-Hyp collagen tripeptide for matrix, stability and enzyme models
- FormLyophilized powder, sealed glass vial
- Mol. weight285.30 g/mol
- Research areasCollagen hydrolysate composition, peptide transport, fibroblast models, glycation, oxidative stress and DPP-IV assays
Overview
Tripeptide-29 is glycyl-L-prolyl-L-hydroxyproline, abbreviated Gly-Pro-Hyp. The motif recurs throughout collagen and is commonly detected among the small peptides produced by collagen hydrolysis. Hydroxyproline distinguishes it from Gly-Pro-Pro and contributes the hydroxyl group central to many collagen-structure and peptide-transport studies.
Research applications span collagen-model stability, uptake and metabolism of collagen-derived peptides, fibroblast responses to hydrolysate fractions, glycation/oxidative-stress assays and in-vitro enzyme work involving DPP-IV. A single purified tripeptide helps determine whether an observation attributed to a complex collagen hydrolysate can actually be reproduced by Gly-Pro-Hyp alone.
The product is supplied as a 200 mg lyophilized vial, CAS 2239-67-0, formula C12H19N3O5 and molecular weight 285.30 g/mol. It is HPLC tested with lot-matched documentation and is intended only for analytical, biochemical and cell-model research—not as a food, supplement, cosmetic or drug.
Identity precision matters for this reagent. Gly-Pro-Hyp is frequently discussed alongside Pro-Hyp and Hyp-Gly, which are different molecules that appear in the same collagen-hydrolysate literature, and hydroxyproline itself is a diastereomeric centre, so catalogue entries should specify 4-hydroxy-L-proline rather than 'Hyp' alone. Supplying the tripeptide as a single purified compound with documented HPLC purity is what allows it to serve as an analytical reference against which mixed hydrolysate fractions can be quantified.
Specifications
Identity
- CAS number
- 2239-67-0
- Molecular formula
- C12H19N3O5
- Molecular weight
- 285.30 g/mol
- Amino acid sequence
- Gly-Pro-Hyp (glycyl-prolyl-hydroxyproline)
- Chain length
- 3 residues
- Peptide class
- Collagen-derived imino-acid-rich tripeptide
Supply & purity
- Form
- Lyophilized powder, sealed glass vial
- Purity
- ≥99% by HPLC; lot-matched COA available
- Solubility
- Water-soluble
Additional detail
- Available size
- 200 mg
- Also known as
- Gly-Pro-Hyp, GPH, collagen tripeptide, Tripeptide-29
- Research areas
- Collagen hydrolysate composition, peptide transport, fibroblast models, glycation, oxidative stress and DPP-IV assays
- Storage
- −20 °C lyophilized; keep sealed and dry
Working out the concentration
The vial sizes above — 200 mg — describe a mass of material, not a strength. Choose a diluent volume, work it out with the reconstitution calculator, and you have the concentration for your own vial of Tripeptide-29 (Gly-Pro-Hyp) plus the volume that carries any mass you want to withdraw.
Where a method is written in molar terms instead, the molecular weight above — 285.30 g/mol — is the input the molarity calculator needs.
Certificate of analysis
Every lot of Tripeptide-29 (Gly-Pro-Hyp) is released against a certificate of analysis: the batch-specific laboratory data for that lot, not a generic document for the compound. The lot number on the certificate matches the lot number printed on the label you receive — the vial label itself, where the product is vialled — so the record and the material it describes can always be reconciled.
Purity: ≥99% by HPLC — the specification for this listing. The figure for the lot you receive is the one reported on that lot’s certificate.
No lot certificate is on display on this page at the moment. That is a publishing state and not a testing one: lots are released and documented as described above whether or not the document is posted here, and we will send what exists for the lot in stock, or for a lot already on your bench, by its number.
Certificates are available on request before you order as well as after, with no account and no charge. Request the certificate for this listing, read the testing and documentation policy in full, or see how to read a peptide COA.
Questions about Tripeptide-29 (Gly-Pro-Hyp)
What is Tripeptide-29?
Tripeptide-29 is the defined three-residue collagen peptide Gly-Pro-Hyp, or glycyl-prolyl-hydroxyproline. It is one of the recurring motifs and digestion products associated with collagen.
Is Tripeptide-29 the same as collagen hydrolysate?
No. A collagen hydrolysate contains many peptides of different lengths and sequences. This vial contains one purified tripeptide, making it useful as an analytical standard or single-agent control.
Why is hydroxyproline important?
Hydroxyproline is characteristic of collagen and affects peptide conformation, enzyme susceptibility and analytical detection. Measuring total hydroxyproline alone, however, cannot prove that intact Gly-Pro-Hyp is present.
What purity documentation is available?
The material is released at ≥99% purity by HPLC with a lot-matched certificate of analysis. Quantitative protocols should account for any counterion or water content shown on the lot documentation.
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