- ≥99% HPLC purity
- Lot-matched certificate of analysis
- Ships next business day, tracked
Vitamin C (Ascorbic Acid) Injection
Ascorbic acid solution, 500 mg/mL in a 30 mL multi-dose vial
- FormClear, colourless to pale straw aqueous solution in a sealed glass vial
- Mol. weight176.12 g/mol
- Research areasCollagen maturation, redox buffering in culture, pharmacologic-ascorbate pro-oxidant models, DNA demethylation and stem-cell studies, iron chemistry
Supplied for laboratory research use only. Not for human or veterinary use, and not intended to diagnose, treat, cure or prevent any disease.
Overview
Biochemically it is a reducing agent and a cofactor. It donates electrons to a family of iron- and copper-dependent enzymes, keeping their metal centres in the reduced state — prolyl and lysyl hydroxylases in collagen maturation, dopamine beta-hydroxylase in catecholamine synthesis, and the 2-oxoglutarate-dependent dioxygenases that include the TET enzymes and the HIF prolyl hydroxylases. That last group is why ascorbate turns up so often in epigenetics and hypoxia-signalling papers.
Specifications
Identity
- CAS number
- 50-81-7
- Molecular formula
- C6H8O6
- Molecular weight
- 176.12 g/mol
Supply & purity
- Form
- Clear, colourless to pale straw aqueous solution in a sealed glass vial
Additional detail
- Concentration
- 500 mg/mL L-ascorbic acid
- Chemical class
- Water-soluble vitamin; six-carbon lactone of a hexonic acid, redox-active
- Chemistry note
- Weak acid; solutions are pH-adjusted, which means part of the content is present as the ascorbate anion
- Enzyme roles
- Prolyl and lysyl hydroxylases, dopamine beta-hydroxylase, 2-oxoglutarate-dependent dioxygenases including TET and HIF-PHD
- Research areas
- Collagen maturation, redox buffering in culture, pharmacologic-ascorbate pro-oxidant models, DNA demethylation and stem-cell studies, iron chemistry
- Oxidation sensitivity
- High — degrades on exposure to air, light, heat and trace transition metals
- Storage
- 2–8 °C, protected from light, tightly stoppered; do not freeze
- Appearance check
- Discard if markedly darkened, cloudy or containing particulates
Questions about Vitamin C (Ascorbic Acid) Injection
How much ascorbic acid is in the vial?
The 30 mL vial is filled at 500 mg/mL, so it contains 15 g of L-ascorbic acid in total. The lot-matched certificate of analysis states the measured assay value alongside identity and pH for that specific batch.
Why does the solution darken over time?
Ascorbate oxidises to dehydroascorbate and then to diketogulonic acid, and the coloured degradation products accumulate. Light, warmth, air and trace transition metals all accelerate it. Marked darkening, cloudiness or particulates mean the vial should be discarded rather than assayed.
Is a certificate of analysis provided?
Yes. Every lot is released with a certificate stating identity, assay concentration, pH and appearance, available on request against the lot number printed on the vial. Assay documentation matters particularly for an easily oxidised compound.
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